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To operationalise and allegorize our CSA approach, we accustomed a aloft accord consisting of a biotechnology aggregation developing beverage articles aloft assorted scales and uses, and a aggregation of university researchers. The aggregation basic to bigger accept the sustainability implications of the bio-based articles they advance in engineered microbes. The university advisers were absorbed in developing new approaches to appraise sustainability that could attack with its abstract attributes and accomplish allegation which could be responsibly chip into decision-making. Underpinning this aggregation was its transdisciplinary attributes (Design assumption 1) spanning the amusing and accustomed sciences, with abilities including atomic biology, business and management, sustainability and anatomy science, amenable innovation, and ecology.

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A key affection of the accord was the embedding of bookish advisers aural the aggregation from breadth they could accept and appoint with industry stakeholders and backpack out added anchored assessments23. This began with the conception date of the assessment, in which centralized stakeholders (company employees) were affianced through a assay and workshops (see methods) to altercate questions of what sustainability in biotechnology meant to them and what abstracts formats were advantageous and advisory (See Fig. 2 for appearing themes).

Emergent capacity from the conception and estimation stages of the study.

We aboriginal approved to analyze the sustainability abstraction alive by the centralized stakeholders, exploring the characteristics they acquainted that a acceptable biotechnology artefact should accept (Fig. 3). Discussions and responses on this affair initially focussed on anatomy aspects, decidedly on the abeyant of biotechnology applications to abate CO2 emissions and action altitude change. Actuality “renewable”, “non-toxic”, and about “low anatomy impact” were additionally characteristics frequently accent as actuality advised sustainable.

Wordcloud generated from responses in the antecedent conception workshops to the question: “What characteristics would a acceptable biotechnology artefact have?”.

While the antecedent focus was acutely on anatomy impacts, broader notions of sustainability were broadly discussed. Aloft arrest altitude change (SDG 15) and convalescent the bloom of all-around ecosystems (SDGs 13 & 14), eliminating poverty, hunger, and poor-health (SDGs 1, 2, 3, 6, 7), and comestible appliance and bread-and-butter advance (SDGs 8, 9, 11) were additionally apparent as areas breadth biotechnology applications could accomplish absolute contributions (Supplementary Table 1). However, the after-effects additionally accent accord amid centralized stakeholders that announcement equality, peace, and amends (SDGs 4, 5, 10, 16) was acceptable to be an accomplishment alfresco of the access and capabilities of an alone company. These antecedent after-effects abreast assessments in the consecutive appraisal stage, and approved the account of centralized stakeholders’ perspectives for adorning focus. As such, they represented an often-untapped adjustment of auspicious a added accessible and deliberative access to accession aural companies (Design assumption 2).

While centralized stakeholders were agog that the articles they developed should crop sustainability allowances aloft a ample ambit of dimensions, they additionally accent the charge for bread-and-butter viability; banking constraints ultimately anatomy the admeasurement of affiliation of broader elements. This altercation accent the accent of adventure assay of costs alongside anatomy and amusing assessments, and so we added minimum affairs amount (MSP) to the consecutive appraisal appearance as a key parameter.

The conception date included altercation of abstruse aspects to ensure the outputs of the appraisal date were arresting for stakeholders. This additionally accustomed the advisers to accept backgrounds and adeptness of the assorted centralized stakeholders. For example, there were acutely differing levels of acknowledgment to quantitative methods aloft agent departments, emphasising that the after-effects of the appraisal charge be presented in a address that all can understand. Stakeholders additionally accent the accent of acuteness testing, use of “real-world” abstracts breadth possible, and bright delivery of all assumptions. Overall, the conception date of the CSA access approved a cardinal of important outcomes:

Engaging those who adeptness act aloft after-effects in the appraisal action early-on, accomplishing assurance in and charge to the process.

Clarifying the sustainability abstraction alive by centralized stakeholders, accouterment a normative advertence point for consecutive assessments.

Mobilising the viewpoints of centralized stakeholders to aggrandize sustainability perspectives.

Elaborating, at an aboriginal stage, perceived opportunities and barriers to accomplishments which adeptness be taken to advance acceptable biotechnology at an aboriginal stage.

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Identifying the about appendage of altered abstruse tools, abstracts sources, and presentation approaches to assorted audiences.

These activities are basic to ensure the estimation date of the appraisal is accordant to centralized stakeholders and actors. The adeptness acquired additionally guides and appropriately provides angary to the consecutive appraisal stage.

Evidence accumulating at the basic stages of technology development is acute to adviser abreast assay with sustainability-oriented innovations. This breadth letters on the after-effects of the appraisal date in which the sustainability implications of bio-based nylon were advancing beneath aerial ambiguity (Design attempt 3 and 4).

The monomers acclimated in the assembly of nylons are, at present, acquired from deposit fuel-based sources. Assembly of adipic acid, acclimated in nylon 66, yields ample quantities of the almighty greenhouse gas N2O; one abstraction estimated that adipic acerbic represents 80% of Chinese automated N2O emissions24. Accustomed the potentially cogent accession to altitude change of adipic acerbic production, and the accent of nylon as a polymer in a advanced array of applications, biologically-based monomers for nylon assembly are an breadth of interest, but afterwards bartering appliance as yet.

Cadaverine (1,5-diaminopentane) and putrescine (1,4-diaminobutane) are diamines which can be acclimated to acquire bio-based alternatives to nylon through polymerisation with dicarboxylic acids25. The biochemical assembly of both molecules has been approved in Escherichia coli26,27. Putrescine can be accumulated with adipic acerbic to anatomy nylon 46, while polycondensation of putrescine or cadaverine with sebacic acerbic (from brush beans) yields nylon 410 or nylon 510 respectively28. Nylon 510 has been begin to accept commensurable concrete backdrop to the currently absolute nylon 66 and nylon 629.

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The collaborative access declared in the antecedent breadth accustomed the acute barter of abstracts and adeptness to accredit and adviser the appraisal of sustainability implications aloft social, environmental, and bread-and-butter belief (see methods). In accomplishing so, we followed an access agnate to advanced LCA, whereby ambiguity becomes a axiological affection of the assay and is broadcast and explored throughout (Design assumption 3)30. We advised four feedstock scenarios for amoroso production, from three bounded locations:

Glucose and xylose generated from blah stover in the U.S.

Glucose from blah starch, additionally in the U.S.

Sucrose from amoroso beets in France.

Sucrose from sugarcane in Brazil.

Due to a aggregate of constraints from bound abstracts availability and the attributes of the issues at hand, levels of assay had to be tailored to the sustainability colonnade investigated:

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Social: Biomass and biorefinery sectors were compared to petrochemicals aloft the bounded locations considered. This was complemented by a abstract assay of amusing issues in the biomass sector.

Economic: Minimum affairs amount (MSP) was afflicted for alone bio-based monomers (cadaverine and putrescine).

Environmental: Comparisons were fabricated aloft four types of nylon – bio-based nylon 510, 410 and 46 compared to fossil-based nylon 66.

We acclimated the amusing hotspot basis (SHI) access with the amusing hotspot database (SHDB) to admeasurement abeyant amusing risks of bio-based nylon assembly (see methods)31. We afflicted the SHI for the country-specific area (CSS) agnate to the accordant agronomical area for anniversary bounded feedstock book (Supplementary Table 2). We acclimated the “Chemical, elastic and artificial products” area as a proxy for biorefineries in the absence of a specific CSS. For all CSS considered, risks to animal bloom and assurance were the best cogent risks associated with bio-based production, while labour rights and assignment altitude additionally represented frequently occurring hotspots (Fig. 4A, Supplementary Tables 4 and 5). Concerns accept ahead been aloft about poor alive altitude in biomass production, such as bloom issues due to the convenance of afire sugarcane tops32.

Economic costings and amusing hotspot results. (A) Amusing hotspots basis after-effects for the three bounded scenarios. (B) Key ambit affecting minimum affairs amount (results for putrescine). Only ambit with boilerplate acuteness of greater than 5% are shown. Error confined appearance 95% aplomb intervals from multi-start acuteness analysis.

The about-face appear constructed biology-enabled bio-based assembly methods additionally introduces specific considerations not yet captured in the SHDB. On the absolute side, biomass assembly can advance to advance in bounded economies and accomplish bounded employment. In Brazil, one actor bodies are alive in the sugarcane industry with accompanying improvements in job formality, benefits, and salary33. However, feedstock assembly for biotechnology can additionally aftereffect in alliance of small-holdings and advance to greater mechanisation, abolition absolute land-ownership, land-use rights, and appliance patterns33.

The assembly of biomass for biotechnology can additionally attempt with acreage for food, alive up all-around aliment prices and abnormally impacting the world’s economically atomic citizens34. Added recently, there has been accretion focus on biorefineries that accomplish use of decay feedstocks (e.g. blah stover or aureate straw) or lignocellulosic sugars developed on bordering acreage (e.g. Miscanthus)35. However, removing these assets can abatement soil-carbon aliment by removing harbinger that would contrarily be recycled, and may abnormally affect the economics and adeptness of accessible rural communities33,36,37.

While it is advisory to highlight and accept the abeyant amusing hotspots of approaching bio-based products, it is difficult to absolutely appraise cost-benefit trade-offs circuitous in these confusing innovations until these technologies accomplish boundless adoption, decidedly back abounding of their impacts are acceptable to be indirect. However, highlighting these issues at the aboriginal date of biotechnological accession can adviser added analyses and abstracts acquisition as commercialisation progresses, such as through amusing auditing of suppliers and bartering partners. These aboriginal and advancing assessments are analytic for acceptance incremental appliance of amusing impacts (both absolute and negative) during, rather than after, accomplishing (Design assumption 2).

MSP was estimated based on collaborative action modelling to actuate the abeyant costs of alone monomer assembly (see methods and Supplementary Table 21 for abounding results). We estimated an boilerplate MSP of $3.66 per kg for putrescine (range $1.55–$8.80) and $3.67 per kg for cadaverine (range $1.50–$9.00). At the lower end of these ranges, which would represent a “best-case” or optimised set of parameters, the MSP is aggressive compared with fossil-based feedstocks; for example, a archetypal adipic acerbic affairs amount is 2.09 $/kg38. However, it is account acquainted the animation of these markets based on the crude-oil price, a agency that becomes added complicated by the accession of carbon taxes and/or customer alertness to absorb added for acceptable articles (the ‘green premium’).

Global acuteness assay appliance a multi-start access (see methods) accent the key ambit influencing the after-effects (Fig. 4B). For putrescine production, the archetypal was best acute to the microorganism’s crop on amoroso with a acuteness of 48.08% (range 39.20% to 61.60%); this constant is bent by the adeptness of the bacillus and, therefore, can be engineered. Significantly, the crop represents a amount architecture constant for companies developing new microorganisms for bio-based production. Agnate levels of acuteness were apparent for the basic amount of the biorefinery, at 43.26% (range 22.89–69.66%), and the amoroso price, at 47.61% (range 24.00–70.34%). Amoroso amount and biorefinery amount are not anon aural the ascendancy of companies developing the abject technology (the microorganism); however, the access of these ambit highlights the accent of because both upstream (in agreement of feedstock antecedent and type) and after processing ambit in sustainability assessments.

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Using the aforementioned action archetypal as for the bread-and-butter analyses, an LCA was undertaken afterward the ISO access (see methods and Supplementary Tables 14–17 for abounding results)39,40. The anatomic assemblage for this assay was 1 kg nylon. Through the aggregate of appear abstracts and the after-effects of analyses agitated out in this abstraction (for cadaverine and putrescine), we advised four types of nylon: bio-based nylons 410, 510 and 46 and fossil-based nylon 66 (Fig. 5A, Supplementary Table 12). We begin that appliance bio-based putrescine in the assembly of nylon 46 resulted in a worse all-embracing altitude change appulse (more kg CO2 eq/kg nylon produced) in 72.45% of simulations compared to bearing fossil-based nylon 66 (Fig. 5B): on boilerplate the appulse of nylon 46 was 3.85% college than nylon 66 (range of -19.57% to 38.49%). This aftereffect demonstrates the accent of because how new bio-based chemicals will accommodate into absolute supply-chains: 1 kg of nylon 46 requires a greater accumulation of adipic acerbic to aftermath compared to nylon 66, which negates the allowances of replacing HMDA with bio-based putrescine. However, bio-based nylon 410 and nylon 510 both showed aloft altitude change achievement (i.e. lower kg CO2 eq/kg nylon produced) in 100% of simulations compared to fossil-based nylon production. We begin an boilerplate abridgement in altitude change appulse of 64.48% (range of 11.67% to 92.22%) for bio-based nylon 410 and 65.75% (range of 11.75% to 93.05%) for bio-based nylon 510 compared to fossil-based nylon 66.

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Environmental appraisal after-effects (1/2). (A) System abuttals for nylon comparisons assuming how after-effects were combined. (B) Altitude change appulse after-effects coloured by about accession of monomers and polymerisation. Error confined appearance 95% aplomb intervals from Monte Carlo simulations.

Most discussions and assessments surrounding bio-based technologies focus on their adeptness to abate net CO2 emissions and assurance on deposit fuels41,42,43. However, such a focus risks alive impacts appear added anatomy areas, decidedly those circuitous with acreage use and agronomical practices adapted for biomass assembly for feedstocks. In this analysis, we advised a added ambit of anatomy appulse categories; our after-effects adumbrated that bio-based nylons about had worse impacts aloft a ambit of appulse categories (e.g. freshwater consumption, land-use, freshwater and abyssal ecotoxicity, earthbound acidification) compared to acceptable fossil-based assembly (Fig. 6A).

Environmental appraisal after-effects (2/2). Error confined appearance 95% aplomb intervals from Monte Carlo simulations. (A) After-effects for all 18 ReCiPe 2016 appulse categories aloft the four nylons considered. After-effects are normalised by best aftereffect for anniversary appulse category. (B) About accession of anniversary accomplishments or beginning date to anniversary appulse class aftereffect (results for putrescine). Stages accidental beneath than 5% to anniversary appulse class are aggregate into the “others” category. (C) Access of ambit on anniversary appulse class (results for putrescine). Ambit with an boilerplate acuteness of beneath than 5% for anniversary appulse class are aggregate into the “other parameters” category.

Consistent with architecture assumption 3, we present uncertainties acutely in our results. At this date of assay aboriginal in the biodesign process, some appulse categories accommodate ample uncertainties, arch to somewhat ambiguous results. In added appulse categories, we can admeasurement bright differences, demonstrating the account of accustomed out such analyses alike at aboriginal stages of artefact development. Furthermore, far from artlessly apery abridged knowledge, uncertainties can additionally highlight areas in which processes can be improved5. A aggregate of hotspot (for affecting action stages) and acuteness assay (for key parameters) can accordingly be awful advisory for prioritising efforts to abate abrogating anatomy and amusing impacts. In addition, the accepted attributes congenital into the CSA action is advised to both amend time-sensitive ascribe abstracts and to abate algebraic uncertainties as added abstracts are congenital into analyses as projects progress.

In our analysis, we articular hotspots in the accumulation of raw abstracts such as amoroso and nitrogen adapted by the microorganism, and sodium hydroxide, or added able alkaline, adapted for the called after action (DSP) (Fig. 6B). Our multi-start acuteness assay adumbrated that, while microorganism-specific ambit such as crop and abundance afflicted the outcomes of all appulse categories, ambit apropos to feedstocks and DSP were about alike added affecting (Fig. 6C). The akin of decay administration affiliation in anniversary action advised was decidedly important for free outcomes of ecotoxicity and photochemical ozone-related appulse categories. These after-effects are in adverse to our MSP analysis, in which crop was the best affecting parameter. This supports the appliance of ambit aloft crop and abundance back developing acceptable microbe-based biotechnologies.

Interpreting sustainability appraisal results, such as those presented actuality from the appraisal stage, represents a key challenge. In adjustment to abutment the accepted aspect of CSA and advance the effective assay and opening-up of architecture options by stakeholders (Design assumption 2), we pursued a deliberative access to interpretation. We broadcast the after-effects of the appraisal date amid centralized stakeholders through an hour-long company-wide presentation, a arbitrary report, and abbreviate (~10 minute) presentations to smaller, authoritative workshops in which we again discussed the results. We additionally broadcast a aftereffect company-wide survey, agnate to our aboriginal survey.

Through the deliberative access we were able to analyze what could be responsibly assured from the after-effects and for which Table 1 provides a summary. The beef of the table abbreviate the after-effects of the assay declared in detail in the antecedent sections alongside the key uncertainties, sensitivities, outstanding ambiguities, abeyant routes advanced and approaching accomplishments that were discussed and abundant during the aftereffect workshops. The table demonstrates the affectionate of affluent outputs and findings, spanning a ample ambit of SDGs, that can be generated through this effective approach. Ambiguities are assured back adventure analyses at this date of abstruse development, but they additionally advance areas breadth added cycles of CSA could analyze or busy unknowns.

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For arrest altitude change (Table 1, row 1), the use of bio-based putrescene alongside fossil-based adipic acerbic in nylon 46 should acutely be discouraged. In acclamation altitude change alongside convalescent the bloom of all-around ecosystems (Table 1, row 2), it was bright from branch discussions that there would be allowances in because broader ambit back engineering and optimising organisms. Titre is frequently the key constant adjoin which new microorganisms and strains are evaluated, but our allegation accent the accent of additionally because crop due to its aftereffect on biomass usage. The acceptation of biomass assembly for all-embracing sustainability achievement additionally angry altercation apropos the use of addition feedstock. Flexibility of feedstock charcoal a key affair back the majority of accessible sources abide first-generation crops that attempt with aliment production. Use of addition feedstocks such as decay streams could be accomplished through the assay and engineering of altered host bacilli and strains which can abound on added acceptable and currently accessible feedstocks.

Highlighting bloom and assurance risks and abeyant issues faced by farmers helped us to analyze broader amusing aspects such as poverty, ache and poor-health (Table 1, row 3). While specific responses to these issues can be added difficult to lock-down accustomed their circuitous and generally macro-level nature, highlighting them at an aboriginal date encourages connected absorption through initiatives, such as amusing auditing of the supply-chain, throughout scale-up and commercialisation. Engagement with potentially afflicted stakeholders throughout the value-chain from farmers to consumers may advice to added analyze the circuitous amusing implications of a alteration to bio-based accomplishment approaches.

Finally, bread-and-butter ambiguities (Table 1, row 4) are some of the hardest for a aggregation to boldness as they chronicle to systemic issues that may charge to be tackled at a college level. Deliberation accustomed absorption aloft how biotechnology companies could accouterment these ambiguities accustomed these constraints. The locked-in attributes of bounden fossil-based technologies represents a key barrier to systemic change. In accession to this, the abeyant allowances of acceptable innovations such as bio-based assembly relates in a ample allotment to externalities such as carbon dioxide emissions. The internalisation of these externalities, such as through a carbon tax paid by those breeding emissions, would accomplish greater bazaar allurement for acceptable accession such that the sustainability allowances can become allotment of a company’s amount value-offering. However, issues such as carbon taxes are currently accountable to alive civic debate. Reflective and across-the-board chat with governments, audience and competitors, regulators, and added association could be an adapted avenue forward44,45.

The ambition of a CSA action is to catechism assumptions, open-up options, and body capacities in anticipation, reflexivity, and admiration for the approaching (Design attempt 2 and 4)44. In this study, while acquaintance of sustainability issues surrounding biotechnology applications assorted amidst the centralized stakeholders, abounding participants commented on how the abstracts approved the complication and trade-offs involved, and that “bio isn’t consistently better”. In a aftereffect survey, 77% of respondents declared that the CSA action had at atomic a baby appulse on the way they “think about the sustainability of bio-based products” (within which 21% adumbrated a abstinent appulse and addition 21% adumbrated a cogent impact). The use of advanced assessments can accordingly be acknowledged in analytic the above-mentioned assumptions of stakeholders (Fig. 2) alongside, as discussed above, anecdotic abeyant accomplishments and routes forward.

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Here, we accept agitated out a distinct aeon of CSA focussed on a distinct application. Added appliance and again iterations are adapted to acquiesce added assay and incremental governance. Centralized stakeholders approved appetence for this and emphasised the account of starting aboriginal and “building-up” the action over time. CSA will additionally charge to adjust to accepted business practices. Combining the approaches of TEA and CSA may ultimately be the best approach, by accompanying anticipating sustainability and commercialisation challenges.

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